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Addgene

Xfz8-pXT7
(Plasmid #32991)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32991 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXT7
  • Backbone manufacturer
    R. Freeman/ S. Sokol
  • Backbone size w/o insert (bp) 3050
  • Vector type
    Xenopus RNA expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Xfz8
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1979
  • Entrez Gene
    fzd8.L (a.k.a. XELAEV_18030715mg, Xfz8, frizzled8, fz8, fzd8, fzd8-a, fzd8-b)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make mRNA, digest with Pst1 and transcribe with T7 RNA polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Xfz8-pXT7 was a gift from Sergei Sokol (Addgene plasmid # 32991 ; http://n2t.net/addgene:32991 ; RRID:Addgene_32991)

  • For your References section:

    A role for Xenopus Frizzled 8 in dorsal development. Itoh K, Jacob J, Y Sokol S. Mech Dev. 1998 Jun;74(1-2):145-57. 10.1016/S0925-4773(98)00076-8 PubMed 9651509
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