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Addgene

Xwnt2b-pXT7
(Plasmid #32988)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32988 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXT7
  • Backbone manufacturer
    R. Freeman/ S. Sokol
  • Backbone size w/o insert (bp) 3000
  • Vector type
    RNA Synthesis and Injections

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Xwnt2b
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1600
  • Entrez Gene
    wnt2b-b (a.k.a. Xwnt-2, Xwnt-2b, Xwnt2, int1l1, irp, wnt-2, wnt-2b, wnt13, wnt2, wnt2-A, xwnt2b)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene quality control sequencing discovered a P159A point mutation. According to the depositing lab, this mutation is not a concern, as the product is functionally active.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Xwnt2b-pXT7 was a gift from Sergei Sokol (Addgene plasmid # 32988 ; http://n2t.net/addgene:32988 ; RRID:Addgene_32988)

  • For your References section:

    Xwnt-2b is a novel axis-inducing Xenopus Wnt, which is expressed in embryonic brain. Landesman Y, Sokol SY. Mech Dev. 1997 May;63(2):199-209. 10.1016/S0925-4773(97)00041-5 PubMed 9203142
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