pBABE-2XFLAG,2XHA-CyclinF-puro
(Plasmid
#32976)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE-puro
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Backbone manufacturerWeinberg Lab
- Backbone size w/o insert (bp) 5200
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCYCLIN F
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SpeciesH. sapiens (human)
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Entrez GeneCCNF (a.k.a. FBX1, FBXO1, FTDALS5)
- Promoter 5'LTR
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Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pBABE-5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-2XFLAG,2XHA-CyclinF-puro was a gift from Michele Pagano (Addgene plasmid # 32976 ; http://n2t.net/addgene:32976 ; RRID:Addgene_32976) -
For your References section:
SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation. D'Angiolella V, Donato V, Vijayakumar S, Saraf A, Florens L, Washburn MP, Dynlacht B, Pagano M. Nature. 2010 Jul 1;466(7302):138-42. 10.1038/nature09140 PubMed 20596027