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mCherry-CyclinF
(Plasmid #32975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CYCLIN F
  • Species
    H. sapiens (human)
  • Entrez Gene
    CCNF (a.k.a. FBX1, FBXO1, FTDALS5)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCherry (N terminal on backbone)
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site MluI (destroyed during cloning)
  • 5′ sequencing primer mCherry-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-CyclinF was a gift from Michele Pagano (Addgene plasmid # 32975 ; http://n2t.net/addgene:32975 ; RRID:Addgene_32975)

  • For your References section:

    SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation. D'Angiolella V, Donato V, Vijayakumar S, Saraf A, Florens L, Washburn MP, Dynlacht B, Pagano M. Nature. 2010 Jul 1;466(7302):138-42. 10.1038/nature09140 PubMed 20596027
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