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Addgene

pcDNA3-FLAG-CyclinF-ΔF
(Plasmid #32973)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CYCLIN F
  • Species
    H. sapiens (human)
  • Mutation
    F-box mutations: L35A and P36A
  • Entrez Gene
    CCNF (a.k.a. FBX1, FBXO1, FTDALS5)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This mutation impairs the binding to Skp1 and prevents the formation of a functional SCF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FLAG-CyclinF-ΔF was a gift from Michele Pagano (Addgene plasmid # 32973 ; http://n2t.net/addgene:32973 ; RRID:Addgene_32973)

  • For your References section:

    SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation. D'Angiolella V, Donato V, Vijayakumar S, Saraf A, Florens L, Washburn MP, Dynlacht B, Pagano M. Nature. 2010 Jul 1;466(7302):138-42. 10.1038/nature09140 PubMed 20596027
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