3XMEF2-luc
(Plasmid #32967)

• Purpose: Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter
• Depositing Lab: Ron Prywes
• Publication: Prywes Lab Plasmids (unpublished)

Backbone

• Vector backbone: pGL3-promoter modified
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MEF2 binding sites
• Species: H. sapiens (human)
• Entrez Gene: MEF2A (a.k.a. ADCAD1, RSRFC4, RSRFC9, mef2)
• Promoter: MEF2
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: Luc_N_Rev

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was cloned from pFOS WT-GL3 (Addgene #11983), where the human c-fos promoter was removed and replaced with 4 MEF2 sites. See author's map for restriction site/MCS details.