pMEF2D-3XFlag
(Plasmid
#32965)
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PurposeFor mammalian expression of mouse MEF2D with a 3x FLAG tag on the C-terminus.
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep3XFlag-CMV-14
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 6310
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMEF2D
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SpeciesM. musculus (mouse)
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Entrez GeneMef2d
- Promoter CMV
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Tags
/ Fusion Proteins
- Flag (C terminal on backbone)
- Flag (C terminal on backbone)
- Flag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site None (unknown if destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer CMV-Fwd
- 3′ sequencing primer hGH-pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMEF2D-3XFlag was a gift from Ron Prywes (Addgene plasmid # 32965 ; http://n2t.net/addgene:32965 ; RRID:Addgene_32965)