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PurposeFor mammalian expression of human LZIP with a 3x FLAG tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32960 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3XFlag-CMV-7.1
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLZIP
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SpeciesH. sapiens (human)
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Entrez GeneCREB3 (a.k.a. LUMAN, LZIP, sLZIP)
- Promoter CMV
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Tags
/ Fusion Proteins
- Flag (N terminal on backbone)
- Flag (N terminal on backbone)
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site None (unknown if destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3XFlag-LZIP was a gift from Ron Prywes (Addgene plasmid # 32960 ; http://n2t.net/addgene:32960 ; RRID:Addgene_32960)