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Addgene

pCGN-S2P-WT
(Plasmid #32957)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32957 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCGN
  • Backbone manufacturer
    M. Tanaka
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S2P
  • Species
    H. sapiens (human)
  • Entrez Gene
    MBTPS2 (a.k.a. FLJ32174, IFAP, KFSDX, S2P)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCGN-S2P-WT was a gift from Ron Prywes (Addgene plasmid # 32957 ; http://n2t.net/addgene:32957 ; RRID:Addgene_32957)

  • For your References section:

    ER stress regulation of ATF6 localization by dissociation of BiP/GRP78 binding and unmasking of Golgi localization signals. Shen J, Chen X, Hendershot L, Prywes R. Dev Cell. 2002 Jul . 3(1):99-111. 10.1016/S1534-5807(02)00203-4 PubMed 12110171
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