pEGFP-ATF6
(Plasmid #32955)

• Purpose: For visualization of GFP-ATF6 in mammalian cells.
• Depositing Lab: Ron Prywes
• Publication: Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-52.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATF6
• Species: H. sapiens (human)
• Mutation: M67L and M313I
• Entrez Gene: ATF6 (a.k.a. ACHM7, ATF6A, ATP6alpha)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry