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pcDNA6-rbN1 S77A
(Plasmid #32945)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32945 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6/HisC
  • Backbone manufacturer
    Invitrogene
  • Backbone size w/o insert (bp) 5200
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLc9a3r1
  • Alt name
    NHERF
  • Species
    rabbit
  • Insert Size (bp)
    1200
  • Mutation
    Change Serine77 to Alanine
  • GenBank ID
    u19815
  • Entrez Gene
    SLC9A3R1
  • Promoter CMV
  • Tags / Fusion Proteins
    • his (N terminal on backbone)
    • T7 tag (N terminal on insert)
    • partial FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer t7
  • 3′ sequencing primer bgh
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NHERF-1 was cut from peGFP-rbNHERF-1 (ID#31636) constructs.Part of the peGFP vector is present at the 3' end. Serine77 was mutated to Alanine

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA6-rbN1 S77A was a gift from Edward Weinman (Addgene plasmid # 32945 ; http://n2t.net/addgene:32945 ; RRID:Addgene_32945)

  • For your References section:

    PTH transiently increases the percent mobile fraction of Npt2a in OK cells as determined by FRAP. Weinman EJ, Steplock D, Cha B, Kovbasnjuk O, Frost NA, Cunningham R, Shenolikar S, Blanpied TA, Donowitz M. Am J Physiol Renal Physiol. 2009 Dec;297(6):F1560-5. Epub 2009 Sep 30. 10.1152/ajprenal.90657.2008 PubMed 19794105
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