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PurposeRetroviral expression of mouse Brn2
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs
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Backbone manufacturer4800
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBrn2
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SpeciesM. musculus (mouse)
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Entrez GenePou3f2 (a.k.a. 9430075J19Rik, A230098E07Rik, Brn-2, Brn2, OTF-7, Otf7, oct-7)
- Promoter 5'LTR
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-Brn2 was a gift from Kevin Eggan (Addgene plasmid # 32925 ; http://n2t.net/addgene:32925 ; RRID:Addgene_32925) -
For your References section:
Conversion of mouse and human fibroblasts into functional spinal motor neurons. Son EY, Ichida JK, Wainger BJ, Toma JS, Rafuse VF, Woolf CJ, Eggan K. Cell Stem Cell. 2011 Sep 2;9(3):205-18. 10.1016/j.stem.2011.07.014 PubMed 21852222