pCGT-Sos Cat
(Plasmid
#32923)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32923 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCGT
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSOS1
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SpeciesH. sapiens (human)
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Insert Size (bp)1455
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MutationContains aa 564-1049 (catalytic domain)
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Entrez GeneSOS1 (a.k.a. GF1, GGF1, GINGF, HGF, NS4, SOS-1)
- Promoter CMV
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Tag
/ Fusion Protein
- T7 epitope (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCGT-Sos Cat was a gift from Dafna Bar-Sagi (Addgene plasmid # 32923 ; http://n2t.net/addgene:32923 ; RRID:Addgene_32923) -
For your References section:
Regulation of ras signaling dynamics by Sos-mediated positive feedback. Boykevisch S, Zhao C, Sondermann H, Philippidou P, Halegoua S, Kuriyan J, Bar-Sagi D. Curr Biol. 2006 Nov 7;16(21):2173-9. 10.1016/j.cub.2006.09.033 PubMed 17084704