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Addgene

HA-Sos2
(Plasmid #32921)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32921 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCGN
  • Backbone manufacturer
    M. Tanaka
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SOS2
  • Species
    H. sapiens (human)
  • Entrez Gene
    SOS2 (a.k.a. NS9, SOS-2)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-Sos2 was a gift from Dafna Bar-Sagi (Addgene plasmid # 32921 ; http://n2t.net/addgene:32921 ; RRID:Addgene_32921)

  • For your References section:

    Differential interactions of human Sos1 and Sos2 with Grb2. Yang SS, Van Aelst L, Bar-Sagi D. J Biol Chem. 1995 Aug 4;270(31):18212-5. 10.1074/jbc.270.31.18212 PubMed 7629138
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