EGFP-Tubulin.K40Q
(Plasmid #32912)

• Depositing Lab: Tso-Pang Yao
• Publication: Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-26.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tubulin
• Alt name: TUBA1B
• Alt name: K-ALPH-1
• Species: H. sapiens (human)
• Mutation: K40Q
• Entrez Gene: TUBA1B (a.k.a. K-ALPHA-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: BGH-Rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the acetylation-mimicking mutant.