pcDNA3β-FLAG-CBP-LD-HA
(Plasmid #32906)

• Depositing Lab: Tso-Pang Yao
• Publication: Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C/EBP
• Alt name: creb binding protein
• Species: M. musculus (mouse)
• Mutation: L1435A/D1436A
• Entrez Gene: Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: BGH-Rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the catalytic deficient mutant.