pCS2-hSecurin
(Plasmid #32903)

• Depositing Lab: Marc Kirschner
• Publication: Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

Backbone

• Vector backbone: pCS2+
• Backbone manufacturer: RZPD
• Backbone size w/o insert (bp): 4000
• Modifications to backbone: Modified with N-term ZZ-Tev4 (two IgG binding domains of protein A (ZZ), followed by four TEV-protease cleavage sequences).
• Vector type: Mammalian Expression ; in vitro translation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Securin
• Species: H. sapiens (human)
• Entrez Gene: PTTG1 (a.k.a. EAP1, ECRAR, HPTTG, PTTG, TUTR1)
• Promoter: simian CMV IE94
• Tag / Fusion Protein:
  • ZZ-Tev4 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

N-terminal tag is two IgG binding domains of protein A (ZZ), followed by four TEV-protease cleavage sequences.