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Addgene

Mef2D-shRNA
(Plasmid #32900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSuper
  • Backbone manufacturer
    OligoEngine
  • Backbone size w/o insert (bp) 3176
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mef2d
  • gRNA/shRNA sequence
    5’- GTAGCTCTCTGGTCACTCC -3’
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Mef2d
  • Promoter H1-RNA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Can be used in conjunction with pcDNA3-rMEF2D (Addgene #32902) which is mutated so as to not be targeted by this shRNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mef2D-shRNA was a gift from Michael Greenberg (Addgene plasmid # 32900 ; http://n2t.net/addgene:32900 ; RRID:Addgene_32900)

  • For your References section:

    Activity-dependent regulation of MEF2 transcription factors suppresses excitatory synapse number. Flavell SW, Cowan CW, Kim TK, Greer PL, Lin Y, Paradis S, Griffith EC, Hu LS, Chen C, Greenberg ME. Science. 2006 Feb 17;311(5763):1008-12. 10.1126/science.1122511 PubMed 16484497
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