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Addgene

pEGFP-H1.5
(Plasmid #32898)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H1.5
  • Alt name
    HIST1H1B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    681
  • GenBank ID
    X83509 P16401
  • Entrez Gene
    H1-5 (a.k.a. H1, H1.5, H1B, H1F5, H1s-3, HIST1H1B)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-H1.5 was a gift from Michael Hendzel (Addgene plasmid # 32898 ; http://n2t.net/addgene:32898 ; RRID:Addgene_32898)

  • For your References section:

    H1 family histones in the nucleus. Control of binding and localization by the C-terminal domain. Th'ng JP, Sung R, Ye M, Hendzel MJ. J Biol Chem. 2005 Jul 29;280(30):27809-14. Epub 2005 May 23. 10.1074/jbc.M501627200 PubMed 15911621
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