pEGFP-H1.1
(Plasmid #32894)

• Depositing Lab: Michael Hendzel
• Publication: Th'ng et al J Biol Chem. 2005 Jul 29;280(30):27809-14. Epub 2005 May 23.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Histone H1.1
• Alt name: HIST1 H1A
• Species: H. sapiens (human)
• Insert Size (bp): 648
• GenBank ID: X57130 Q02539
• Entrez Gene: H1-1 (a.k.a. H1.1, H1A, H1F1, HIST1, HIST1H1A)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry