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Addgene

p4979 pcDNA4-E6AP III L502P
(Plasmid #32892)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32892 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA4
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E6AP
  • Species
    H. sapiens (human)
  • Mutation
    L502P
  • Entrez Gene
    UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6xHis Express (N terminal on backbone)
    • T7-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site EcoR V (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4979 pcDNA4-E6AP III L502P was a gift from Peter Howley (Addgene plasmid # 32892 ; http://n2t.net/addgene:32892 ; RRID:Addgene_32892)
  • For your References section:

    Biochemical analysis of Angelman syndrome-associated mutations in the E3 ubiquitin ligase E6-associated protein. Cooper EM, Hudson AW, Amos J, Wagstaff J, Howley PM. J Biol Chem. 2004 Sep 24;279(39):41208-17. Epub 2004 Jul 19. 10.1074/jbc.M401302200 PubMed 15263005