p4978 pcDNA4-E6AP III E550L
(Plasmid
#32891)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32891 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA4
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE6AP
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SpeciesH. sapiens (human)
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MutationE550L
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Entrez GeneUBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
- Promoter CMV
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Tags
/ Fusion Proteins
- 6xHis Express (N terminal on backbone)
- T7-tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoR V (destroyed during cloning)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p4978 pcDNA4-E6AP III E550L was a gift from Peter Howley (Addgene plasmid # 32891 ; http://n2t.net/addgene:32891 ; RRID:Addgene_32891) -
For your References section:
Biochemical analysis of Angelman syndrome-associated mutations in the E3 ubiquitin ligase E6-associated protein. Cooper EM, Hudson AW, Amos J, Wagstaff J, Howley PM. J Biol Chem. 2004 Sep 24;279(39):41208-17. Epub 2004 Jul 19. 10.1074/jbc.M401302200 PubMed 15263005