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Addgene

FLAG-Matr3 delRRM2
(Plasmid #32882)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32882 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag2B
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MATR3 Matrin3
  • Alt name
    MPD2; VCPDM; MGC9105; KIAA0723; DKFZp686K0542; DKFZp686K23100
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2544
  • Mutation
    deleted amino acids 497-566
  • GenBank ID
    NM_001194954.1
  • Entrez Gene
    MATR3 (a.k.a. ALS21, MPD2, VCPDM)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    no
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that the depositor sequence is for the full length Matr3, not the deletion.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-Matr3 delRRM2 was a gift from Yossi Shiloh (Addgene plasmid # 32882 ; http://n2t.net/addgene:32882 ; RRID:Addgene_32882)

  • For your References section:

    Matrin 3 binds and stabilizes mRNA. Salton M, Elkon R, Borodina T, Davydov A, Yaspo ML, Halperin E, Shiloh Y. PLoS One. 2011;6(8):e23882. Epub 2011 Aug 17. 10.1371/journal.pone.0023882 PubMed 21858232
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