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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-Tag2B
- Backbone size w/o insert (bp) 4300
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMATR3 Matrin3
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Alt nameMPD2; VCPDM; MGC9105; KIAA0723; DKFZp686K0542; DKFZp686K23100
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SpeciesH. sapiens (human)
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Insert Size (bp)2544
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GenBank IDNM_001194954.1
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Entrez GeneMATR3 (a.k.a. ALS21, MPD2, VCPDM)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byno
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-MATR3 was a gift from Yossi Shiloh (Addgene plasmid # 32880 ; http://n2t.net/addgene:32880 ; RRID:Addgene_32880) -
For your References section:
Matrin 3 binds and stabilizes mRNA. Salton M, Elkon R, Borodina T, Davydov A, Yaspo ML, Halperin E, Shiloh Y. PLoS One. 2011;6(8):e23882. Epub 2011 Aug 17. 10.1371/journal.pone.0023882 PubMed 21858232