PP-ClpR
(Plasmid
#32876)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32876 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET15-MHL
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Backbone manufacturerStructural Genomics Consortium
- Backbone size w/o insert (bp) 7737
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameClpR
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Alt namePDB: 3SNW
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Alt namePF14_0348
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SpeciesPlasmodium Falciparum
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Entrez GenePF3D7_1436800 (a.k.a. PF3D7_1436800, PF14_0348)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB: 3SNW.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PP-ClpR was a gift from Cheryl Arrowsmith (Addgene plasmid # 32876 ; http://n2t.net/addgene:32876 ; RRID:Addgene_32876)