HECW2
(Plasmid
#32875)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerStructural Genomics Consortium (Addgene plasmid 26096)
- Backbone size w/o insert (bp) 7325
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHECW2
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Alt namePDB: 2LFE
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Alt nameHECT, C2 and WW domain containing E3 ubiquitin protein ligase 2, N-terminal domain
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SpeciesH. sapiens (human)
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Insert Size (bp)360
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Entrez GeneHECW2 (a.k.a. NDHSAL, NEDL2)
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Tag
/ Fusion Protein
- His tag (with TEV cleavage) (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB: 2LFE. http://www.thesgc.org/structures/2LFE/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HECW2 was a gift from Cheryl Arrowsmith (Addgene plasmid # 32875 ; http://n2t.net/addgene:32875 ; RRID:Addgene_32875)