GRM3 (3SM9)
(Plasmid
#32870)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32870 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepFHMSP-LIC-N
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Backbone manufacturerStructural Genomics Consortium (Addgene plasmid 26095)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGRM3
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Alt namePDB: 3SM9
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Alt namemetabotropic glutamate receptor 3, extracellular ligand binding domain
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SpeciesH. sapiens (human)
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Insert Size (bp)1437
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Entrez GeneGRM3 (a.k.a. GLUR3, GPRC1C, MGLUR3, mGlu3)
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Tags
/ Fusion Proteins
- Honeybee melittin Signal sequence (N terminal on backbone)
- His tag with TEV cleavage (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Polyhedrin-fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB: 3SM9. http://www.thesgc.org/structures/3SM9/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GRM3 (3SM9) was a gift from Cheryl Arrowsmith (Addgene plasmid # 32870 ; http://n2t.net/addgene:32870 ; RRID:Addgene_32870)