PP-MAPK2 (3RP9)
(Plasmid
#32862)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerStructural Genomics Consortium (Addgene plasmid 26096
- Backbone size w/o insert (bp) 7325
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMAPK2
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Alt namePDB: 3RP9
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Alt namemitogen-activated protein kinase (25.m01780)
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SpeciesToxoplasma gondii
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Insert Size (bp)1278
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Mutationamino acids 123-548 only, P421L
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Tag
/ Fusion Protein
- His tag with TEV cleavage (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB: 3RP9. http://www.thesgc.org/structures/3RP9/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PP-MAPK2 (3RP9) was a gift from Cheryl Arrowsmith (Addgene plasmid # 32862 ; http://n2t.net/addgene:32862 ; RRID:Addgene_32862)