EGFP-talin1 rod
(Plasmid #32855)

• Depositing Lab: Anna Huttenlocher
• Publication: Simonson et al J Immunol. 2006 Dec 1;177(11):7707-14.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: BD Clontech
• Backbone size w/o insert (bp): 4749
• Modifications to backbone: C1 vector frame shifted by cutting with BglII, blunting, and religating.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Talin 1 Rod aa 434-2541
• Species: M. musculus (mouse)
• Insert Size (bp): 6327
• GenBank ID: CAA39588.1
• Entrez Gene: Tln1 (a.k.a. Tln)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: EGFP-talin1 rod was cloned by removing the talin1 rod cDNA (encoding aa 434-2541) from the pJ6 Ω 7 vector (R. Hynes) and cloning it into the same frame-shifted pEGFP-C1 vector.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that both the full plasmid sequence from the depositing laboratory and Addgene's sequencing results indicate a K673N mutation/SNP when the sequences are compared to GenBank entry NP_035732.2. The depositing laboratory states that this difference is not a concern for the function of the plasmid. The most appropriate reference sequence for the Talin1 insert in the plasmid is GenBank accession number CAA39588.1.