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Addgene

pET30 NHERF1
(Plasmid #32854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32854 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet30a+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5422
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Alt name
    NHERF
  • Species
    O. cuniculus (rabbit)
  • Insert Size (bp)
    1200
  • Mutation
    none
  • GenBank ID
    U19815
  • Entrez Gene
    SLC9A3R1
  • Promoter T7
  • Tags / Fusion Proteins
    • his (N terminal on insert)
    • S16 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site V=EcoRV I =BssH1 (destroyed during cloning)
  • 3′ cloning site V=EcoRV i=EcoR1 (destroyed during cloning)
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pET 30a vector cut with EcoRV
NHERF from SK-cut with BSSH II and ECoRI.
Both filled in and blunt ligated.

insert at bp 5217-6918
aa 1-358 BP#5076 ATG
leader protein = 49 +9 from cDNA not usually translated
EK cuts 43 AA off sequence
nherf tranlates 5244

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30 NHERF1 was a gift from Edward Weinman (Addgene plasmid # 32854 ; http://n2t.net/addgene:32854 ; RRID:Addgene_32854)

  • For your References section:

    Structure-function of recombinant Na/H exchanger regulatory factor (NHE-RF). Weinman EJ, Steplock D, Tate K, Hall RA, Spurney RF, Shenolikar S. J Clin Invest. 1998 May 15;101(10):2199-206. 10.1172/JCI204 PubMed 9593775
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