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Addgene

DCX-RFP
(Plasmid #32851)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32851 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDsRed2-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DCX
  • Alt name
    doublecortin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1110
  • Entrez Gene
    DCX (a.k.a. DBCN, DC, LISX, SCLH, XLIS)
  • Promoter CMV
  • Tag / Fusion Protein
    • DsRed2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer DsRed1-N
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DCX-RFP was a gift from Joseph Gleeson (Addgene plasmid # 32851 ; http://n2t.net/addgene:32851 ; RRID:Addgene_32851)
  • For your References section:

    Lis1 and doublecortin function with dynein to mediate coupling of the nucleus to the centrosome in neuronal migration. Tanaka T, Serneo FF, Higgins C, Gambello MJ, Wynshaw-Boris A, Gleeson JG. J Cell Biol. 2004 Jun 7. 165(5):709-21. 10.1083/jcb.200309025 PubMed 15173193