pACAGW-oChiEFtdTomato-AAV
(Plasmid #32846)

• Purpose: AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.
• Depositing Lab: Rory McQuiston
• Publication: Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371/journal.pone.0045717. Epub 2012 Sep 20.

Backbone

• Vector backbone: pACAGW-ChR2-Venus-AAV
• Backbone manufacturer: Addgene plasmid 20071
• Backbone size w/o insert (bp): 4885
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 20 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: 2YT media for 20hours
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: oChiEF
• Species: Chlamydomonas reinhardtii
• Insert Size (bp): 1077
• Promoter: CAGG
• Tag / Fusion Protein:
  • tdTomato (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R Tsien lab, UCSD
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note this plasmid has a I170V in channelopsin 1. The changed amino acid improves the protein’s function.