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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32841 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQCXIH
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 7800
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTAZ
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Alt nameWWTR1
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SpeciesH. sapiens (human)
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Entrez GeneWWTR1 (a.k.a. TAZ)
- Promoter CMV/MSV
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Tag
/ Fusion Protein
- Myc
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer LNCX (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQCXIH-myc-hTAZ was a gift from Kunliang Guan (Addgene plasmid # 32841 ; http://n2t.net/addgene:32841 ; RRID:Addgene_32841) -
For your References section:
TAZ promotes cell proliferation and epithelial-mesenchymal transition and is inhibited by the hippo pathway. Lei QY, Zhang H, Zhao B, Zha ZY, Bai F, Pei XH, Zhao S, Xiong Y, Guan KL. Mol Cell Biol. 2008 Apr;28(7):2426-36. Epub 2008 Jan 28. 10.1128/MCB.01874-07 PubMed 18227151