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Addgene

HA-TAZ-S89A
(Plasmid #32840)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32840 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-HA
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TAZ
  • Alt name
    WWTR1
  • Species
    H. sapiens (human)
  • Mutation
    S89A
  • Entrez Gene
    WWTR1 (a.k.a. TAZ)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-TAZ-S89A was a gift from Kunliang Guan (Addgene plasmid # 32840 ; http://n2t.net/addgene:32840 ; RRID:Addgene_32840)

  • For your References section:

    TAZ promotes cell proliferation and epithelial-mesenchymal transition and is inhibited by the hippo pathway. Lei QY, Zhang H, Zhao B, Zha ZY, Bai F, Pei XH, Zhao S, Xiong Y, Guan KL. Mol Cell Biol. 2008 Apr;28(7):2426-36. Epub 2008 Jan 28. 10.1128/MCB.01874-07 PubMed 18227151
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