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HA-AMOT p130 deltaEYLI
(Plasmid #32826)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32826 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-HA
  • Backbone manufacturer
    Guan lab
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AMOT p130
  • Species
    H. sapiens (human)
  • Mutation
    E5D, deletion of last 4 amino acids (EYLI)
  • Entrez Gene
    AMOT
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E5D is present in all Guan lab AMOT p130 cDNAs. According to the depositing lab, it has no effect on function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-AMOT p130 deltaEYLI was a gift from Kunliang Guan (Addgene plasmid # 32826 ; http://n2t.net/addgene:32826 ; RRID:Addgene_32826)

  • For your References section:

    Angiomotin is a novel Hippo pathway component that inhibits YAP oncoprotein. Zhao B, Li L, Lu Q, Wang LH, Liu CY, Lei Q, Guan KL. Genes Dev. 2011 Jan 1;25(1):51-63. 10.1101/gad.2000111 PubMed 21205866
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