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Addgene

OTV-dDAT
(Plasmid #32812)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32812 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Oocyte Transcription Vector: OTV-2L8
  • Vector type
    Oocyte Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dDAT
  • Alt name
    drosophila dopamine transporter
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    DAT (a.k.a. Dmel_CG8380, CG8380, DmDAT, Dmel\CG8380, dDAT, fmn)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The dDAT coding sequence is flanked by a Xenopus b-globin 5' UTR and a 3'UTR with a poly A signal and tail.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OTV-dDAT was a gift from Susan Amara (Addgene plasmid # 32812 ; http://n2t.net/addgene:32812 ; RRID:Addgene_32812)
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