Smad3-shRNA
(Plasmid
#32806)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSIREN-DNR-DsRed
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6600
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Vector typeMammalian Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSmad3
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gRNA/shRNA sequencen/a
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SpeciesM. musculus (mouse)
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Entrez GeneSmad3 (a.k.a. Madh3)
- Promoter hU6
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Tag
/ Fusion Protein
- DsRed (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site None (unknown if destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer U6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Primers used to generate shRNA:
Upper: 5’–
GATCCACCTGAGTGAAGATGGAGATTCAAGAGATCTCCATCTTCACTCAGGTTTTTTTACGCGTG–3’
Lower: 3’– AATTCACGCGTAAAAAAACCTGAGTGAAGATGGAGATCTCTTGAATCTCCATCTTCACTCAGGTG-5'
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Smad3-shRNA was a gift from Vicki Boussiotis (Addgene plasmid # 32806 ; http://n2t.net/addgene:32806 ; RRID:Addgene_32806)