pRSETB-S28 histone 3 phosphorylation reporter
(Plasmid
#32788)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSETB
- Backbone size w/o insert (bp) 5500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCFP-14-3-3tau-H3 peptide-YFP
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Insert Size (bp)1686
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GenBank IDAY422821.1
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Tags
/ Fusion Proteins
- CFP (N terminal on insert)
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Xpress-F
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSETB-S28 histone 3 phosphorylation reporter was a gift from Alice Ting (Addgene plasmid # 32788 ; http://n2t.net/addgene:32788 ; RRID:Addgene_32788) -
For your References section:
A genetically encoded fluorescent reporter of histone phosphorylation in living cells. Lin CW, Ting AY. Angew Chem Int Ed Engl. 2004 May 24;43(22):2940-3. 10.1002/anie.200353375 PubMed 15170310