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Addgene

pcDNA3-S28 histone 3 phosphorylation reporter-NLS
(Plasmid #32787)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32787 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CFP-14-3-3tau-H3 peptide-YFP
  • Insert Size (bp)
    2244
  • Tags / Fusion Proteins
    • CFP (N terminal on insert)
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-S28 histone 3 phosphorylation reporter-NLS was a gift from Alice Ting (Addgene plasmid # 32787 ; http://n2t.net/addgene:32787 ; RRID:Addgene_32787)

  • For your References section:

    A genetically encoded fluorescent reporter of histone phosphorylation in living cells. Lin CW, Ting AY. Angew Chem Int Ed Engl. 2004 May 24;43(22):2940-3. 10.1002/anie.200353375 PubMed 15170310
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