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Addgene

pGEM-7Zf-TYR
(Plasmid #32786)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32786 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM 7Zf
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    In vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tyrosinase
  • Species
    H. sapiens (human)
  • Mutation
    I181M
  • GenBank ID
    Y00819.1
  • Entrez Gene
    TYR (a.k.a. ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing results identified an I181M mutation in tyrosinase sequence. This mutation was not intentional, but this is the exact plasmid as described in the associated article listed below.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-7Zf-TYR was a gift from Ruth Halaban (Addgene plasmid # 32786 ; http://n2t.net/addgene:32786 ; RRID:Addgene_32786)

  • For your References section:

    Translation rate of human tyrosinase determines its N-linked glycosylation level. Ujvari A, Aron R, Eisenhaure T, Cheng E, Parag HA, Smicun Y, Halaban R, Hebert DN. J Biol Chem. 2001 Feb 23;276(8):5924-31. Epub 2000 Nov 7. 10.1074/jbc.M009203200 PubMed 11069924
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