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Addgene

ZM2
(Plasmid #32784)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ZM
  • Vector type
    Cre/Lox ; Floxed targeting vector for Trypanosome
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    T7rnap

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To lower the toxicity, we modified pyrFE upstream sequences from TACATTGTTTAAGTCGCTTCAACCTTGTTTCTTTTTTTTTTCCTTTTCTTCTTCTTCGCTATCTGATTGAGTTTTCATACTAAGTTGGATAAG" to TACATTGTTTAAGTCGCTTCAACCTTGTTTCTTTTTTTTTTCCTTATCTGATTGAGTTTTCATACTAAGTTGGAT

There are a few mismatches between Addgene's quality control sequence and the provided reference sequence from the depositing lab. The mismatches should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZM2 was a gift from George Cross (Addgene plasmid # 32784 ; http://n2t.net/addgene:32784 ; RRID:Addgene_32784)
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