ptyrEGFP-TYR
(Plasmid #32782)

• Depositing Lab: Ruth Halaban
• Publication: Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97(11):5889-94.

Backbone

• Vector backbone: pEGFP-N
• Backbone manufacturer: Clontech
• Modifications to backbone: CMV promoter was replaced with 2500bp Tyrosinase promoter from mouse
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tyrosinase
• Species: H. sapiens (human)
• Entrez Gene: TYR (a.k.a. ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3)
• Promoter: Tyrosinase
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: EGFP_N and hTYR-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CMV promoter in pEGFP-N vector was replaced with 2500bp of tyrosinase endogenous promoter from mouse.

Please note that while the tyrosinase promoter is mouse, the sequence of the tyrosinase insert in this plasmid is human.