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Addgene

μ2-HA-D176A
(Plasmid #32754)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32754 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    μ2 subunit of clathrin adaptor complex AP-2
  • Alt name
    μ2
  • Species
    R. norvegicus (rat)
  • Mutation
    D176A
  • Entrez Gene
    Ap2m1 (a.k.a. Ap50, Clapm1, mu2)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NB: The HA tag (YPYDVPDYALE) was inserted within the protein between residues 236 and 237.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    μ2-HA-D176A was a gift from Alexander Sorkin (Addgene plasmid # 32754 ; http://n2t.net/addgene:32754 ; RRID:Addgene_32754)

  • For your References section:

    Inhibition of the receptor-binding function of clathrin adaptor protein AP-2 by dominant-negative mutant mu2 subunit and its effects on endocytosis. Nesterov A, Carter RE, Sorkina T, Gill GN, Sorkin A. EMBO J. 1999 May 4;18(9):2489-99. 10.1093/emboj/18.9.2489 PubMed 10228163
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