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Addgene

EGFR-GFP Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: SacII and SacII+SnaBI double digest.

We expected the following band sizes for this digest: 8.4kb for SacII and 4.4 + 4kb for the double digest. We see the expected pattern.