EGFR-GFP
(Plasmid #32751)

• Purpose: Mammalian expression of epidermal growth factor receptor (EGFR) fused to EGFP
• Depositing Lab: Alexander Sorkin
• Publication: Carter et al J Biol Chem. 1998 Dec 25;273(52):35000-7.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4672
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFR
• Species: H. sapiens (human)
• Insert Size (bp): 3639
• Entrez Gene: EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Ullrich/Joseph Schelessinger, Yale University Axel Ullrich, Max Planck Institute of Biochemistry
• Articles Citing this Plasmid:
  • 53 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To create the EGFR-GFP fusion (in-frame), the the full-length cDNA of EGFR including 3' and 5' untranslated regions was cloned into pEGFP-N1 as an XbaI/HindIII fragment. This is EGFR-wt. The 3' end of the EGFR cDNA including STOP codon and untranslated region was removed by PCR and digestion. The 3' primer generated a new SstII (SacII) site.