pT-CA-GFP-IRES-mLumin
(Plasmid #32749)

• Depositing Lab: Jeanne Hardy
• Publication: Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-86. Epub 2011 May 10.

Backbone

• Vector backbone: pT-CD8-IRES-SCN2B
• Backbone manufacturer: Dr. Alfred George, Vanderbilt University
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: CA-GFP
• Insert Size (bp): 854
• Mutation: S65T
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R

Gene/Insert 2

• Gene/Insert name: mLumin
• Alt name: mKate2-S158A
• Insert Size (bp): 700
• Mutation: S158A

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: IRES-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CA-GFP sequence is GFP fused with DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL at the C-terminus