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Addgene

Myc-tagged human full length TCF4E pcDNA3
(Plasmid #32738)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32738 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TCF4E
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Entrez Gene
    TCF7L2 (a.k.a. TCF-4, TCF4)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a Q27R mutation in Addgene's quality control sequence. This is a SNP, natural mutation in the cDNA from Dr Clevers.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc-tagged human full length TCF4E pcDNA3 was a gift from Frank McCormick (Addgene plasmid # 32738 ; http://n2t.net/addgene:32738 ; RRID:Addgene_32738)
  • For your References section:

    Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. Tetsu O, McCormick F. Nature. 1999 Apr 1;398(6726):422-6. 10.1038/18884 PubMed 10201372