Myc-tagged human full length TCF4E pcDNA3
(Plasmid #32738)

• Depositing Lab: Frank McCormick
• Publication: Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TCF4E
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Entrez Gene: TCF7L2 (a.k.a. TCF-4, TCF4)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a Q27R mutation in Addgene's quality control sequence. This is a SNP, natural mutation in the cDNA from Dr Clevers.