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-962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic
(Plasmid #32736)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32736 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCND1 Promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1096
  • Mutation
    -75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion; -27 TCF(3) TTTTGTT deletion; and -15 TCF(4) TGCAAAG deletion
  • Entrez Gene
    CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
  • Promoter -962 CCND1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site Hind3 (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer GLprimer2
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a 1bp mismatch in the sequence that does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    -962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic was a gift from Frank McCormick (Addgene plasmid # 32736 ; http://n2t.net/addgene:32736 ; RRID:Addgene_32736)

  • For your References section:

    Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. Tetsu O, McCormick F. Nature. 1999 Apr 1;398(6726):422-6. 10.1038/18884 PubMed 10201372
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