-962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic
(Plasmid
#32734)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 32734 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGL3Basic
-
Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
-
Vector typeLuciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCCND1 promoter
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1096
-
Mutation-75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deletion
-
Entrez GeneCCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
- Promoter -962 CCND1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site Hind3 (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer GLprimer2 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
-962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic was a gift from Frank McCormick (Addgene plasmid # 32734 ; http://n2t.net/addgene:32734 ; RRID:Addgene_32734) -
For your References section:
Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. Tetsu O, McCormick F. Nature. 1999 Apr 1;398(6726):422-6. 10.1038/18884 PubMed 10201372
Map uploaded by the depositor.