hTLR3-pcDNA3
(Plasmid #32712)

• Depositing Lab: Saumen Sarkar
• Publication: Zhu et al J Immunol. 2010 May 15;184(10):5768-76. Epub 2010 Apr 9.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TLR3
• Species: H. sapiens (human)
• Entrez Gene: TLR3 (a.k.a. CD283, IIAE2, IMD83)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Preprototrypsin (N terminal on insert)
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the E127G point mutation does not affect the function of this plasmid.